Detection and analysis of hairpin II, an essential metastable structural element in viroid replication intermediates
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چکیده
منابع مشابه
Detection and analysis of hairpin II, an essential metastable structural element in viroid replication intermediates.
In (-)-stranded replication intermediates of the potato spindle tuber viroid (PSTVd) a thermodynamically metastable structure containing a specific hairpin structure (HP II) has been proposed to be essential for viroid replication. In the present work a method was devised allowing the direct detection of the HP II structure in vitro and in vivo using a biophysical approach. An RNA oligonucleoti...
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Molecular hybridization by the Northern blot technique in combina t ion with 12SI-labeled PSTV (+) RNA and S2P-labeled PSTV cDNA as probes has been applied to d e t e c t v i r o i d s p e c i f i c s e q u e n c e s in h e a l t h y and v i r o i d ( P S T V ) i n f e c t e d t o m a t o plants . Conditions are described which allow d i f f e r e n t i a t i o n of (+) and (-) viroid sequences...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2002
ISSN: 1362-4962
DOI: 10.1093/nar/gkf454